Created by: Hande Küçük McGinty, Janice Kranz, Joseph Ostrow, Alex Clark, Yu Lin, Saminda Abeyruwan, Uma Vempati, Kunie Sakurai, Vance Lemmon, Ubbo Visser, John Turner, Stephan Schurer*
Contact: Stephan Schurer stephan dot schurer at gmail dot com
The quality of a biological macromolecule (DNA, RNA or protein), which could either be unmodified' (native) or modified, e.g., methylated, phosphorylated, etc.
A quality of a DNA or RNA residue that has a methyl group attached to it.
The phenotype of an organism that is most common in nature and was unmodified.
It is the quality which allows a microorganism to survive after exposure to one or more antibiotics.
Fusion proteins or chimeric proteins are created through the joining of two or more cDNAs which encode different proteins. Upon expression, a single polypeptide containing each of the proteins results. Generally, the protein of interest is tagged with a reporter protein, e.g. luciferase, GFP, etc, whose expression could easily be monitored.
A quality of the genetic material (DNA or RNA) that harbors a change (mutation), where one or more nucleotides are lost (spontaneously) or removed (intentionally).
A physical quality which is equal to the distance between repeating units of a wave pattern (from PATO). For fluorescence measurements, it is the wavelength at which the fluorophore is excited and the wavelength at which it emits fluorescence. In the case of absorbance, it is the wavelength at which light is absorbed by a biological entity or a dye.
For fluorescence measurements, it is the wavelength at which a fluorophore is excited
For fluorescence measurements, it is the wavelength at which an excited fluorophore emits fluorescence.
In absorbance measurements, it is the wavelength at which light is absorbed by a biological entity or a dye
A quality of the genetic material (DNA or RNA) that harbors a change (mutation), where one or more nucleotides are added either spontaneously (within the cell) or intentionally (experimentally).
A quality of the genetic material (DNA or RNA) that harbors a change (mutation), where one nucleotide is replaced by a different nucleotide either spontaneously (within the cell) or intentionally (experimentally).
Mutation is any alteration in a gene, which may be disease causing or a normal variant. Mutations are introduced into the genes and subsequently, introduced into cells for expression. Different types of mutations include missense, nonsense, deletions, insertions, etc.
A quality of a protein that harbors a change (mutation), where a portion of the protein towards the carboxyl-terminal is missing either spontaneously (within the cell) or intentionally (experimentally).
A quality of an amino acid residue that has a moiety (e.g., phosphate, carbohydrate) attached to it after translation.
A quality of a protein that harbors a change (mutation), where either one or more amino acids are replaced by different ones to study the role of those amino acids in the function of the protein.
The phenotype of an organism that has been derived by genetic modification.
Cell line culturing describes the growth mode of a cell line, whether it grows attached to the culture dish (adherent) or floating (suspension) in the culture medium or partially attached (mixed adherent and suspension).
A quality of a protein of being the predominant form in an organism.
A quality of a DNA or RNA residue that has a group (e.g., methyl) attached to it after transcription.
A quality of an amino acid residue that has a carbohydrate moiety attached to it.
The different varieties or classes of antibodies, which differ in their properties and biological functions.
Bandwidth is the range of wavelength of light that it allows to pass through the filter, which is either excitation or emission type.
A quality of a protein that harbors a variation from the native protein (called canonical form), which could arise due to alternative promoter usage, alternative splicing, alternative translation initiation and ribosomal frameshifting.