Created by: Hande Küçük McGinty, Janice Kranz, Joseph Ostrow, Alex Clark, Yu Lin, Saminda Abeyruwan, Uma Vempati, Kunie Sakurai, Vance Lemmon, Ubbo Visser, John Turner, Stephan Schurer*
Contact: Stephan Schurer stephan dot schurer at gmail dot com
The title of the kit/biologicals used in the assay.
Kinase-Glo Plus Luminescent Kinase Assay
CellTiter-Glo Luminescent Cell Viability Assay
BacTiter-Glo Microbial Cell Viability Assay
Amplex Red Glucose/Glucose Oxidase Assay Kit
IRI prefix change from BAO_02000 to BAO_00012 in January 2017
ELISA cAMP Competition Assay using cAMP-Screen Direct System
Vybrant MTT Cell Proliferation Assay Kit
Phospho(Thr180/Tyr182)/Total p38 Assay Whole Cell Lysate Kit
Cellomics BrdU and Ki67 Cell Proliferation Kit
Cellomics Cytoskeletal Rearrangement Kit
Cellomics Multiparameter Cytotoxicity 1 Kit
Cellomics Multiplexed NFκB and c-Jun Activation Kit
Cellomics Multiplexed p53 and p21 Detection Kit
Alphascreen second messenger IP1 detection kit
LanthaScreen Co-Factor Interaction Assay
PathScan Apoptosis and Proliferation Multiplex IF Kit
PathScan EGF Receptor Activation Multiplex IF Kit
PathScan Multi-Target HCA DNA Damage Kit
PathScan Multi-Target HCA Stress and Apoptosis Kit
PathScan Signaling Nodes Multiplex IF Kit
CytoTox-Glo Cytotoxicity Assay (Promega) uses a luminogenic peptide substrate of luciferase, the AAF-Glo Substrate, to measure dead-cell protease activity, which is released from cells that have lost membrane integrity.
The CAT ELISA is used to quantitatively measure CAT expression in eukaryotic cells that are transfected with a plasmid bearing a CAT-encoding reporter gene.
ACTOne cAMP biosensor assay (BD Biosciences; CodexBIosolutions)
This assay directly measures the intracellular concentration of the second messenger cAMPin living cells, in real-time. It works with cell lines that contain a proprietary exogenous Cyclic Nucleotide-Gated (CNG) channel (BD Biosciences, sold to CodexBiosolutions; both in Maryland). The channel is activated by elevated intracellular levels of cAMP, resulting in ion flux (detectable by calcium-responsive dyes) and cell membrane depolarization which can be detected with a fluorescent membrane potential (MP) dye.
CellTiter-Blue Cell Viability Assay (Promega)
The CellTiter-Blue Cell Viability Assay provides a homogeneous, fluorescent method for monitoring cell viability. The assay is based on the ability of living cells to convert a redox dye (resazurin) into a fluorescent end product (resorufin)
Gal-Screen Beta-Galactosidase Reporter Gene Assay system
The Gal-Screen assay system combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of _-galactosidase reporter enzyme.
QuantiTect SYBR Green PCR Kits (Qiagen/Thermo-Fisher)
Real-time PCR (or RT-PCR) for quantitation of DNA (or RNA, respectively) using the fluorescent dye SYBR green that binds dsDNA and emits fluorescence only when bound.
TaqMan Real-Time PCR assays (Thermo-Fisher)
Real-time PCR (or RT-PCR) for quantitation of DNA (or RNA, respectively) using a pair of unlabeled PCR primers and a 'TaqMan' fluorogenic probe labeled on the 5' end and minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3' end, to detect target as it accumulates during PCR.
Ultra-Glo (Promega) is a thermostable recombinant luciferase that can be coupled with different luciferin-based substrates to generate a luminescent signal proportional to the substrate abundance.
Luciferase-based ('Glo') Assay System (Promega)
An assay system (Promega) based on the bioluminescent enzyme luciferase's catalysis of the substrate luciferin to generate light, adapted for multiple applications, such as cell viability, apoptosis, or kinase, cytochrome P450, or proteasome activity.
A colorimatric assay for cell viability/ proliferation based on the stable tetrazolium salt WST-1, which is cleaved to a soluble formazan by a complex cellular mechanism that occurs primarily at the cell surface. This bioreduction is largely dependent on the glycolytic production of NAD(P)H in viable cells.
Reverse Transcription Kit (Applied Biosystems or ABI)
A homogeneous bead-based proximity assay (alpha = 'amplified luminescent proximity homogeneous assay'). Binding of molecules captured on donor and acceptor beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. Ligands on the bead allow for detection of many different types of molecules.
A solid phase sandwich ELISA to meaasure human cytochrome c in cell lysates
OptEIA human IL-2 ELISA (BD Biosciences)
TRANSCREENER ADP2 FP Kinase Assay (BellBrook Labs)
A fluorescence polarization (FP) assay that uses an antibody against ADP and a tracer to measure the progress of any enzyme that produces ADP
Polar Screen PPARg Competitor Assay, Green (Invitrogen)
QUANTI-Blue is a colorimetric enzyme assay to measure alkaline phosphatase activity.
The ToxiLighte BioAssay Kit is a bioluminescent cytolysis assay kit that measures release of the enzyme, adenylate kinase (AK), from damaged cells.
TransAM Kit for NF-kB Family (Active Motif)
TransAM Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TRANSCREENER AMP2/GMP2 FP Assay (BellBrook Labs)
A fluorescence polarization (FP) assay that uses an antibody against AMP or GMP and a tracer to measure the progress of any enzyme that produces AMP or GMP
A homogeneous, bioluminescent assay to measure caspase-6 activity.
CDP-Star alkaline phosphatase assay system
CDP-Star is a chemiluminescent substrate of alkaline phosphatase, compatible with both membrane- and solution-based assays. Sold by multiple vendors.
Sensolyte 520 HCV Protease Assay Kit (AnaSpec)
An assay kit for measuring hepatitits C virus (HCV) NS3/4A protease activity using a 5-FAM/QXL520 fluorescence resonance energy transfer (FRET) peptide. Called 'Enzolyte' as well as 'Sensolyte'.
EnzyChrom Aspartate Transaminase Assay Kit (BioAssay Systems)
An absorbance-based assay system for measuring aspartate transaminase (known as serum glutamic oxaloacetic transaminase (GOT) or aspartate aminotransferase (ASAT/AAT)), based on the quantification of oxaloacetate produced by AST. In this assay, oxaloacetate and NADH are converted to malate and NAD by the enzyme malate dehydrogenase. The decrease in NADH absorbance at 340 nm is proportionate to AST activity.
FluoZin-2 AM fluorescent assay of zinc concentration
FluoZin-2 AM is a cell-permeant (acetoxymymethyl (AM)) fluorescent indicator designed to detect Zn2+ concentrations that are present in synaptic vesicles and released in response to electrical stimulation or excitotoxic agonists. (Sold by Molecular Probes, Themro-Fisher)
Phospho-ERK1/2 (Thr202/Tyr204) Cellular Assay Kit (Cisbio)
This homogeneous (HTRF) phospho-ERK assay is based on a TR-FRET sandwich immunoassay format comprising two specific monoclonal anti-pERK1/2 antibodies (recognizing the phosphorylated residue (Thr202/Tyr204), one labeled with Eu3+-cryptate (donor) and the other labeled with d2 (acceptor). The proximity of donor and acceptor leads to a fluorescent TR-FRET signal; the signal intensity is proportional to the substrate phosphorylation. Also called 'HTRF Cellul'ERK Kit'.
IMAP Assay of phosphoproteins (Molecular Devices)
IMAP (Immobilized Metal Ion Affinity Particle) technology is a non-antibody-based assay for protein kinases in which a fluorescently labeled peptide substrate that is phosphorylated by a kinase is captured on trivalent metal-containing nanoparticles. IMAP can be adapted for either FP or TR-FRET detection.
This Insulin ELISA kit is an FDA registered in vitro diagnostic tool for the quantification of human insulin. It uses a dual-monoclonal antibody sandwich ELISA format, typically in a 96-well or other microplate format, with colorimetric (OD 450 nm) detection.
An assay to monitor protein ubiquitination, using ubiquitin labeled with the LanthaScreen terbium chelate, in combination with a FRET acceptor such as fluorescein-ubiquitin or a fluorescein-labeled antibody.
LanthaScreen Tb-Streptavidin Binding Assay
A terbium-labeled streptavidin used in combination with a fluorescent molecule to detect interaction via a TR-FRET assay.
PathHunter U2OS Keap1-NRF2 Nuclear Translocation Assay (DiscoverX)
An assay system employing enzyme fragment complementation (two fragments of the beta-galactosidase enzyme), applied in U2OS cells engineered to express two appropriately tagged proteins. Inhibition of Keap1-NRF2 interaction results in Nrf2 nuclear translocation, and susbequent complementation and formation of a functional enzyme, detected using a chemiluminescent substrate of beta-galactosidase.
qScript One-step Fast qRT-PCR kit (QuantaBioSciences)
A general kit for use in all types of qRT-PCR reactions
Renilla-Glo luciferase assay system (Promega)
A bioluminescent assay sytem for detecting sea pansy (Renilla reniformis) luciferase, an alternative to the firefly (Photinus pyralis) systems.
Ub-CHOP2-Reporter-Kit (LifeSensors Inc.)
An assay for deubiquitination activity, employing a reporter enzyme fused to ubiquitin (making the reporter catalytically inactive). Following cleavage of the Ub-reporter system by the isopeptidase, the free reporter can act upon its fluorescent substrate.
CellTiter-Fluor Cell Viability Assay Kit
The CellTiter-Fluor Cell Viability Assay measures the relative number of viable cells in a population. The assay is based on measurement of a conserved and constitutive protease activity within live cells and therefore serves as a biomarker of cell viability. The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant, peptide substrate (Gly-Phe-AFC). The substrate enters intact cells, where it is cleaved by the live-cell protease activity to generate a fluorescent signal proportional to the number of living cells. The live-cell protease becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium. (description adapted from vendor Promega)