BioAssay Ontology
type: Ontology
license: https://creativecommons.org/licenses/by-sa/4.0/
version info: 2.7.2
label: BAO
comment: Created by: Hande Küçük McGinty, Janice Kranz, Joseph Ostrow, Alex Clark, Yu Lin, Saminda Abeyruwan, Uma Vempati, Kunie Sakurai, Vance Lemmon, Ubbo Visser, John Turner, Stephan Schurer*
comment: Contact: Stephan Schurer stephan dot schurer at gmail dot com
date: 8 Jul 2021

steadylite HTS Reagent
type: Class
subclass of: assay kit

assay kit
type: Class
IAO_0000115: The title of the kit/biologicals used in the assay.

Steady-Glo Luciferase Assay System
type: Class
IAO_0000119: PubChem AID:
IAO_0000116: Date from BAE: 6/1/2016
subclass of: Luciferase-based ('Glo') Assay System (Promega)

Proteasome-Glo Chymotrypsin-Like Assay
type: Class
IAO_0000119: PubChem AID:
IAO_0000116: Date from BAE: 6/1/2016
subclass of: Luciferase-based ('Glo') Assay System (Promega)

PKLight
type: Class
subclass of: assay kit

P450-Glo CYP3A4 Screening System
type: Class
IAO_0000119: PubChem AID:
IAO_0000116: Date from BAE: 6/1/2016
subclass of: Luciferase-based ('Glo') Assay System (Promega)

P450-Glo CYP2D6 Screening System
type: Class
IAO_0000119: PubChem AID:
IAO_0000116: Date from BAE: 6/1/2016
subclass of: Luciferase-based ('Glo') Assay System (Promega)

P450-Glo CYP2C9 Screening System
type: Class
IAO_0000119: PubChem AID:
IAO_0000116: Date from BAE: 6/1/2016
subclass of: Luciferase-based ('Glo') Assay System (Promega)

P450-Glo CYP2C19 Screening System
type: Class
IAO_0000119: PubChem AID:
IAO_0000116: Date from BAE: 6/1/2016
subclass of: Luciferase-based ('Glo') Assay System (Promega)

P450-Glo CYP1A2 Screening System
type: Class
IAO_0000119: PubChem AID:
IAO_0000116: Date from BAE: 6/1/2016
subclass of: Luciferase-based ('Glo') Assay System (Promega)

ONE-Glo Luciferase Assay System
type: Class
IAO_0000119: PubChem AID:
IAO_0000116: Date from BAE: 6/1/2016
subclass of: Luciferase-based ('Glo') Assay System (Promega)

Kinase-Glo Plus Luminescent Kinase Assay
type: Class
IAO_0000119: PubChem AID:
IAO_0000116: Date from BAE: 6/1/2016
subclass of: Luciferase-based ('Glo') Assay System (Promega)

Kinase-Glo Luminescent Kinase Assay
type: Class
IAO_0000119: PubChem AID:
IAO_0000116: Date from BAE: 6/1/2016
subclass of: Luciferase-based ('Glo') Assay System (Promega)

Dual-Luciferase Reporter Assay System
type: Class
IAO_0000119: PubChem AID:
IAO_0000116: Date from BAE: 6/1/2016
subclass of: Luciferase-based ('Glo') Assay System (Promega)

Chroma-Glo Luciferase Assay System
type: Class
IAO_0000119: PubChem AID:
IAO_0000116: Date from BAE: 6/1/2016
subclass of: Luciferase-based ('Glo') Assay System (Promega)

CellTiter-Glo Luminescent Cell Viability Assay
type: Class
IAO_0000119: PubChem AID:
IAO_0000116: Date from BAE: 6/1/2016
subclass of: Luciferase-based ('Glo') Assay System (Promega)

Caspase Glo 3/7 Assay
type: Class
IAO_0000119: PubChem AID:
IAO_0000116: Date from BAE: 6/1/2016
subclass of: Luciferase-based ('Glo') Assay System (Promega)

Bright-Glo Luciferase Assay System
type: Class
IAO_0000119: PubChem AID:
IAO_0000116: Date from BAE: 6/1/2016
subclass of: Luciferase-based ('Glo') Assay System (Promega)

britelite plus
type: Class
subclass of: assay kit

Beta-Glo Assay System
type: Class
IAO_0000119: PubChem AID:
IAO_0000116: Date from BAE: 6/1/2016
subclass of: Luciferase-based ('Glo') Assay System (Promega)

BacTiter-Glo Microbial Cell Viability Assay
type: Class
IAO_0000119: PubChem AID:
IAO_0000116: Date from BAE: 6/1/2016
subclass of: Luciferase-based ('Glo') Assay System (Promega)

ATPLite Luminescence Assay System
type: Class
subclass of: assay kit

ADP Glo Kinase Assay
type: Class
IAO_0000119: PubChem AID:
IAO_0000116: Date from BAE: 6/1/2016
subclass of: Luciferase-based ('Glo') Assay System (Promega)

Adapta Universal Kinase Assay Kit
type: Class
subclass of: assay kit

ADP Hunter Plus
type: Class
subclass of: assay kit

Amplex Red Glucose/Glucose Oxidase Assay Kit
type: Class
subclass of: assay kit

Calcium 5 Assay Kit
type: Class
subclass of: assay kit

cAMP-screen direct system
type: Class
subclass of: assay kit

Fluo-8 No Wash Calcium Assay Kit
type: Class
subclass of: assay kit

FluxOR Thallium Detection Kit
type: Class
subclass of: assay kit

GeneBLAzer
type: Class
subclass of: assay kit

High Performance PBX Calcium Assay Kit
type: Class
subclass of: assay kit

HTRF cAMP Detection Kit
type: Class
subclass of: assay kit

HTRF cAMP HiRange Detection Kit
type: Class
subclass of: assay kit

HTRF KinEASE-TK
type: Class
subclass of: assay kit

HTScan IKK-beta Kinase Assay Kit
type: Class
subclass of: assay kit

HTScan PKC-beta Kinase Assay Kit
type: Class
subclass of: assay kit

HTScan PKC-theta Kinase Assay Kit
type: Class
subclass of: assay kit

IP-One HTRF Assay
type: Class
subclass of: assay kit

LANCE cAMP Detection Kit
type: Class
subclass of: assay kit

LiveBLAzer FRET - B/G Loading Kit
type: Class
subclass of: assay kit

OptEIA Human IL-8 ELISA Set
type: Class
subclass of: assay kit

PathHunter Beta-Arrestin Assay
type: Class
subclass of: assay kit

Tango GPCR Assay System
type: Class
subclass of: assay kit

Transcreener assay kit
type: Class
comment: IRI prefix change from BAO_02000 to BAO_00012 in January 2017
subclass of: assay kit

Lactate kit
type: Class
comment: IRI prefix change from BAO_02000 to BAO_00012 in January 2017
subclass of: assay kit

TruPoint assay kit
type: Class
comment: IRI prefix change from BAO_02000 to BAO_00012 in January 2017
subclass of: assay kit

xMap kit
type: Class
subclass of: assay kit

ECL western blotting detection kit
type: Class
subclass of: assay kit

FLIPR Calcium 3 Assay Kit
type: Class
subclass of: assay kit

LanthaScreen kinase activity assay kit
type: Class
subclass of: assay kit

lance TR-FRET kinase assay kit
type: Class
subclass of: assay kit

AlphaScreen IgG detection kit
type: Class
subclass of: assay kit

AlphaScreen GST detection kit
type: Class
subclass of: assay kit

AlphaScreen cAMP assay kit
type: Class
subclass of: assay kit

ECL peptide assay
type: Class
subclass of: assay kit

ELISA cAMP Competition Assay using cAMP-Screen Direct System
type: Class
subclass of: assay kit

Vybrant MTT Cell Proliferation Assay Kit
type: Class
subclass of: assay kit

Phospho(Thr180/Tyr182)/Total p38 Assay Whole Cell Lysate Kit
type: Class
subclass of: assay kit

Cellomics Beta-Catenin Activation Kit
type: Class
subclass of: assay kit

Cellomics BrdU and Ki67 Cell Proliferation Kit
type: Class
subclass of: assay kit

Cellomics Caspase3 Activation Kit
type: Class
subclass of: assay kit

Cellomics Caspase9 Activation Kit
type: Class
subclass of: assay kit

Cellomics Cell Motility Kit
type: Class
subclass of: assay kit

Cellomics Cell Viability Kit
type: Class
subclass of: assay kit

Cellomics c-Jun Activation Kit
type: Class
subclass of: assay kit

Cellomics Cytoskeletal Rearrangement Kit
type: Class
subclass of: assay kit

Cellomics Mitotic Index Kit
type: Class
subclass of: assay kit

Cellomics Multiparameter Cytotoxicity 1 Kit
type: Class
subclass of: assay kit

Cellomics Multiplexed NFκB and c-Jun Activation Kit
type: Class
subclass of: assay kit

Cellomics Multiplexed p53 and p21 Detection Kit
type: Class
subclass of: assay kit

Cellomics p38 MAPK Activation Kit
type: Class
subclass of: assay kit

PolarScreen Competitor Assay Kit
type: Class
subclass of: assay kit

HitHunter Calcium No WashPLUS Assay Kit
type: Class
subclass of: assay kit

HitHunter cAMP Assay Kit
type: Class
subclass of: assay kit

AlphaScreen Phosphotyrosine Assay Kit
type: Class
subclass of: assay kit

AlphaScreen cGMP Detection
type: Class
subclass of: assay kit

Alphascreen second messenger IP1 detection kit
type: Class
subclass of: assay kit

HTRF biomarkers
type: Class
subclass of: assay kit

ViaLight Plus kit
type: Class
subclass of: assay kit

QuantiChrom Assay Kit
type: Class
subclass of: assay kit

LanthaScreen Co-Factor Interaction Assay
type: Class
subclass of: assay kit

LanthaScreen Competetive Binding Assay
type: Class
subclass of: assay kit

FLIPR Calcium 5 Assay Kit
type: Class
subclass of: assay kit

FLIPR Membrane Potential Assay Kit
type: Class
subclass of: assay kit

CyQUANT Cell Proliferation Assay Kit
type: Class
subclass of: assay kit

HTRF KinEASE
type: Class
subclass of: assay kit

Fluo-4 Calcium Assay Kit
type: Class
subclass of: assay kit

Calcium Assay Kit
type: Class
subclass of: assay kit

KINOMEscan kit
type: Class
comment: Kit uses for Kinome scan assays
subclass of: assay kit

PathScan Apoptosis and Proliferation Multiplex IF Kit
type: Class
subclass of: assay kit

PathScan EGF Receptor Activation Multiplex IF Kit
type: Class
subclass of: assay kit

PathScan EMT Duplex IF Kit
type: Class
subclass of: assay kit

PathScan Multi-Target HCA DNA Damage Kit
type: Class
subclass of: assay kit

PathScan Multi-Target HCA Stress and Apoptosis Kit
type: Class
subclass of: assay kit

PathScan Signaling Nodes Multiplex IF Kit
type: Class
subclass of: assay kit

StemLight Pluripotency Antibody Kit
type: Class
subclass of: assay kit

CytoTox-Glo (Promega)
type: Class
IAO_0000119: https://www.promega.com/resources/protocols/technical-bulletins/101/cytotox-glo-cytotoxicity-assay-protocol/
IAO_0000116: Date from BAE: 6/22/2016
IAO_0000115: CytoTox-Glo Cytotoxicity Assay (Promega) uses a luminogenic peptide substrate of luciferase, the AAF-Glo Substrate, to measure dead-cell protease activity, which is released from cells that have lost membrane integrity.
IAO_0000119: PubChem AID: 435031
subclass of: Luciferase-based ('Glo') Assay System (Promega)

CAT ELISA (Roche/ Sigma-Aldrich)
type: Class
IAO_0000119: PubChem AID: 624301
IAO_0000116: Date from BAE: 6/1/2016
IAO_0000115: The CAT ELISA is used to quantitatively measure CAT expression in eukaryotic cells that are transfected with a plasmid bearing a CAT-encoding reporter gene.
IAO_0000119: http://www.sigmaaldrich.com/catalog/product/roche/11363727001
subclass of: assay kit

ACTOne cAMP biosensor assay (BD Biosciences; CodexBIosolutions)
type: Class
IAO_0000119: http://www.bdbiosciences.com/ds/ab/others/SF47641.pdf
IAO_0000116: Date from BAE: 6/7/2016
IAO_0000115: This assay directly measures the intracellular concentration of the second messenger cAMPin living cells, in real-time. It works with cell lines that contain a proprietary exogenous Cyclic Nucleotide-Gated (CNG) channel (BD Biosciences, sold to CodexBiosolutions; both in Maryland). The channel is activated by elevated intracellular levels of cAMP, resulting in ion flux (detectable by calcium-responsive dyes) and cell membrane depolarization which can be detected with a fluorescent membrane potential (MP) dye.
IAO_0000119: PubChem AID: 651719
subclass of: assay kit

CellTiter-Blue Cell Viability Assay (Promega)
type: Class
IAO_0000119: PubChem AID: 1063
IAO_0000116: Date from BAE: 5/27/2016
IAO_0000115: The CellTiter-Blue Cell Viability Assay provides a homogeneous, fluorescent method for monitoring cell viability. The assay is based on the ability of living cells to convert a redox dye (resazurin) into a fluorescent end product (resorufin)
IAO_0000119: https://www.promega.com/products/cell-health-and-metabolism/cell-viability-assays/celltiter_blue-cell-viability-assay/
subclass of: assay kit

Gal-Screen Beta-Galactosidase Reporter Gene Assay system
type: Class
IAO_0000119: https://www.thermofisher.com/order/catalog/product/T1028
IAO_0000116: Date from BAE: 6/22/2016
IAO_0000115: The Gal-Screen assay system combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of _-galactosidase reporter enzyme.
IAO_0000119: PubChem AID: 2788
subclass of: assay kit

QuantiTect SYBR Green PCR Kits (Qiagen/Thermo-Fisher)
type: Class
IAO_0000119: https://www.qiagen.com/us/shop/pcr/real-time-pcr-enzymes-and-kits/two-step-qrt-pcr/quantitect-sybr-green-pcr-kits/#orderinginformation
IAO_0000116: Date from BAE: 6/26/2016
IAO_0000115: Real-time PCR (or RT-PCR) for quantitation of DNA (or RNA, respectively) using the fluorescent dye SYBR green that binds dsDNA and emits fluorescence only when bound.
IAO_0000119: PubChem AID: 504907
subclass of: assay kit

TaqMan Real-Time PCR assays (Thermo-Fisher)
type: Class
IAO_0000119: https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-assays.html
IAO_0000116: Date from BAE: 6/13/2016
IAO_0000115: Real-time PCR (or RT-PCR) for quantitation of DNA (or RNA, respectively) using a pair of unlabeled PCR primers and a 'TaqMan' fluorogenic probe labeled on the 5' end and minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3' end, to detect target as it accumulates during PCR.
IAO_0000119: PubChem AID: 720492
subclass of: assay kit

Ultra-Glo Luciferin Detection (Promega)
type: Class
IAO_0000119: PubChem AID: 488901
IAO_0000116: Date from BAE: 6/26/2016
IAO_0000115: Ultra-Glo (Promega) is a thermostable recombinant luciferase that can be coupled with different luciferin-based substrates to generate a luminescent signal proportional to the substrate abundance.
subclass of: Luciferase-based ('Glo') Assay System (Promega)

Luciferase-based ('Glo') Assay System (Promega)
type: Class
IAO_0000119: PubChem AID: 624307
IAO_0000116: Date from BAE: 6/1/2016
IAO_0000115: An assay system (Promega) based on the bioluminescent enzyme luciferase's catalysis of the substrate luciferin to generate light, adapted for multiple applications, such as cell viability, apoptosis, or kinase, cytochrome P450, or proteasome activity.
subclass of: assay kit

WST-1 assay (Roche)
type: Class
IAO_0000119: PubChem AID: 602337
IAO_0000116: Date from BAE: 6/30/2016
IAO_0000115: A colorimatric assay for cell viability/ proliferation based on the stable tetrazolium salt WST-1, which is cleaved to a soluble formazan by a complex cellular mechanism that occurs primarily at the cell surface. This bioreduction is largely dependent on the glycolytic production of NAD(P)H in viable cells.
subclass of: assay kit

Reverse Transcription Kit (Applied Biosystems or ABI)
type: Class
IAO_0000119: PubChem AID: 540282
IAO_0000116: Date from BAE: 7/22/2016
subclass of: assay kit

Alpha Screen Assay Kit (PerkinElmer)
type: Class
IAO_0000119: PubChem AID: 2444
IAO_0000116: Date from BAE: 7/6/2016
IAO_0000115: A homogeneous bead-based proximity assay (alpha = 'amplified luminescent proximity homogeneous assay'). Binding of molecules captured on donor and acceptor beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. Ligands on the bead allow for detection of many different types of molecules.
subclass of: assay kit

Human Cytochrome c ELISA (R&D Systems)
type: Class
IAO_0000119: PubChem AID: 504407
IAO_0000116: Date from BAE: 7/3/2016
IAO_0000115: A solid phase sandwich ELISA to meaasure human cytochrome c in cell lysates
subclass of: assay kit

OptEIA human IL-2 ELISA (BD Biosciences)
type: Class
IAO_0000119: PubChem AID: 1271
IAO_0000116: Date from BAE: 7/14/2016
subclass of: assay kit

TRANSCREENER ADP2 FP Kinase Assay (BellBrook Labs)
type: Class
IAO_0000119: PubChem AID: 1795
IAO_0000116: Date from BAE: 7/6/2016
IAO_0000115: A fluorescence polarization (FP) assay that uses an antibody against ADP and a tracer to measure the progress of any enzyme that produces ADP
subclass of: assay kit

Polar Screen PPARg Competitor Assay, Green (Invitrogen)
type: Class
IAO_0000119: PubChem AID: 504453
IAO_0000116: Date from BAE: 7/22/2016
IAO_0000115: A fluorescence polarization (FP) assay.
subclass of: assay kit

QUANTI-Blue (InvivoGen)
type: Class
IAO_0000119: http://www.invivogen.com/quanti-blue
IAO_0000116: Date from BAE: 7/14/2016
IAO_0000115: QUANTI-Blue is a colorimetric enzyme assay to measure alkaline phosphatase activity.
IAO_0000119: PubChem AID: 1369
subclass of: assay kit

ToxiLight BioAssay Kit (Lonza)
type: Class
IAO_0000119: PubChem AID: 651654
IAO_0000116: Date from BAE: 7/9/2016
IAO_0000115: The ToxiLighte BioAssay Kit is a bioluminescent cytolysis assay kit that measures release of the enzyme, adenylate kinase (AK), from damaged cells.
subclass of: assay kit

TransAM Kit for NF-kB Family (Active Motif)
type: Class
IAO_0000119: https://www.activemotif.com/catalog/634/transam-nf%CE%BAb
IAO_0000116: Date from BAE: 7/14/2016
IAO_0000115: TransAM Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
IAO_0000119: PubChem AID: 1295
subclass of: assay kit

TRANSCREENER AMP2/GMP2 FP Assay (BellBrook Labs)
type: Class
IAO_0000119: PubChem AID: 651607
IAO_0000116: Date from BAE: 7/6/2016
IAO_0000115: A fluorescence polarization (FP) assay that uses an antibody against AMP or GMP and a tracer to measure the progress of any enzyme that produces AMP or GMP
subclass of: assay kit

Caspase-Glo 6 Assay System (Promega)
type: Class
IAO_0000119: PubChem AID: 652277
IAO_0000116: Date from BAE: 9/30/2016
IAO_0000115: A homogeneous, bioluminescent assay to measure caspase-6 activity.
subclass of: Luciferase-based ('Glo') Assay System (Promega)

CDP-Star alkaline phosphatase assay system
type: Class
IAO_0000119: PubChem AID: 518
IAO_0000116: Date from BAE: 9/30/2016
IAO_0000115: CDP-Star is a chemiluminescent substrate of alkaline phosphatase, compatible with both membrane- and solution-based assays. Sold by multiple vendors.
subclass of: assay kit

Sensolyte 520 HCV Protease Assay Kit (AnaSpec)
type: Class
IAO_0000119: http://www.anaspec.com/products/product.asp
IAO_0000116: Date from BAE: 9/30/2016
IAO_0000115: An assay kit for measuring hepatitits C virus (HCV) NS3/4A protease activity using a 5-FAM/QXL520 fluorescence resonance energy transfer (FRET) peptide. Called 'Enzolyte' as well as 'Sensolyte'.
IAO_0000119: PubChem AID: 623964
subclass of: assay kit

EnzyChrom Aspartate Transaminase Assay Kit (BioAssay Systems)
type: Class
IAO_0000119: PubChem AID: 743184
IAO_0000116: Date from BAE: 9/30/2016
IAO_0000115: An absorbance-based assay system for measuring aspartate transaminase (known as serum glutamic oxaloacetic transaminase (GOT) or aspartate aminotransferase (ASAT/AAT)), based on the quantification of oxaloacetate produced by AST. In this assay, oxaloacetate and NADH are converted to malate and NAD by the enzyme malate dehydrogenase. The decrease in NADH absorbance at 340 nm is proportionate to AST activity.
IAO_0000119: https://www.bioassaysys.com/Aspartate-Transaminase-Assay-Kit.html
subclass of: assay kit

FluoZin-2 AM fluorescent assay of zinc concentration
type: Class
IAO_0000119: PubChem AID: 623952
IAO_0000116: Date from BAE: 9/30/2016
IAO_0000115: FluoZin-2 AM is a cell-permeant (acetoxymymethyl (AM)) fluorescent indicator designed to detect Zn2+ concentrations that are present in synaptic vesicles and released in response to electrical stimulation or excitotoxic agonists. (Sold by Molecular Probes, Themro-Fisher)
subclass of: assay kit

Phospho-ERK1/2 (Thr202/Tyr204) Cellular Assay Kit (Cisbio)
type: Class
IAO_0000119: PubChem AID: 624053
IAO_0000116: Date from BAE: 9/30/2016
IAO_0000115: This homogeneous (HTRF) phospho-ERK assay is based on a TR-FRET sandwich immunoassay format comprising two specific monoclonal anti-pERK1/2 antibodies (recognizing the phosphorylated residue (Thr202/Tyr204), one labeled with Eu3+-cryptate (donor) and the other labeled with d2 (acceptor). The proximity of donor and acceptor leads to a fluorescent TR-FRET signal; the signal intensity is proportional to the substrate phosphorylation. Also called 'HTRF Cellul'ERK Kit'.
subclass of: assay kit

IMAP Assay of phosphoproteins (Molecular Devices)
type: Class
IAO_0000119: PubChem AID: 624076
IAO_0000116: Date from BAE: 9/30/2016
IAO_0000115: IMAP (Immobilized Metal Ion Affinity Particle) technology is a non-antibody-based assay for protein kinases in which a fluorescently labeled peptide substrate that is phosphorylated by a kinase is captured on trivalent metal-containing nanoparticles. IMAP can be adapted for either FP or TR-FRET detection.
subclass of: assay kit

insulin ELISA kit (Alpco)
type: Class
IAO_0000119: PubChem AID: 488951
IAO_0000116: Date from BAE: 9/30/2016
IAO_0000115: This Insulin ELISA kit is an FDA registered in vitro diagnostic tool for the quantification of human insulin. It uses a dual-monoclonal antibody sandwich ELISA format, typically in a 96-well or other microplate format, with colorimetric (OD 450 nm) detection.
subclass of: assay kit

LanthaScreen Tb-Ubiquitin Assay
type: Class
IAO_0000119: PubChem AID: 2658
IAO_0000116: Date from BAE: 9/30/2016
IAO_0000115: An assay to monitor protein ubiquitination, using ubiquitin labeled with the LanthaScreen terbium chelate, in combination with a FRET acceptor such as fluorescein-ubiquitin or a fluorescein-labeled antibody.
subclass of: assay kit

LanthaScreen Tb-Streptavidin Binding Assay
type: Class
IAO_0000119: PubChem AID: 588664
IAO_0000116: Date from BAE: 9/30/2016
IAO_0000115: A terbium-labeled streptavidin used in combination with a fluorescent molecule to detect interaction via a TR-FRET assay.
subclass of: assay kit

PathHunter U2OS Keap1-NRF2 Nuclear Translocation Assay (DiscoverX)
type: Class
IAO_0000119: PubChem AID: 651833
IAO_0000116: Date from BAE: 9/30/2016
IAO_0000115: An assay system employing enzyme fragment complementation (two fragments of the beta-galactosidase enzyme), applied in U2OS cells engineered to express two appropriately tagged proteins. Inhibition of Keap1-NRF2 interaction results in Nrf2 nuclear translocation, and susbequent complementation and formation of a functional enzyme, detected using a chemiluminescent substrate of beta-galactosidase.
subclass of: assay kit

qScript One-step Fast qRT-PCR kit (QuantaBioSciences)
type: Class
IAO_0000119: PubChem AID: 623968
IAO_0000116: Date from BAE: 9/30/2016
IAO_0000115: A general kit for use in all types of qRT-PCR reactions
subclass of: assay kit

Renilla-Glo luciferase assay system (Promega)
type: Class
IAO_0000119: PubChem AID: 624133
IAO_0000116: Date from BAE: 9/30/2016
IAO_0000115: A bioluminescent assay sytem for detecting sea pansy (Renilla reniformis) luciferase, an alternative to the firefly (Photinus pyralis) systems.
subclass of: Luciferase-based ('Glo') Assay System (Promega)

Ub-CHOP2-Reporter-Kit (LifeSensors Inc.)
type: Class
IAO_0000119: PubChem AID: 652174
IAO_0000116: Date from BAE: 9/30/2016
IAO_0000115: An assay for deubiquitination activity, employing a reporter enzyme fused to ubiquitin (making the reporter catalytically inactive). Following cleavage of the Ub-reporter system by the isopeptidase, the free reporter can act upon its fluorescent substrate.
subclass of: assay kit

CellTiter-Fluor Cell Viability Assay Kit
type: Class
IAO_0000115: The CellTiter-Fluor Cell Viability Assay measures the relative number of viable cells in a population. The assay is based on measurement of a conserved and constitutive protease activity within live cells and therefore serves as a biomarker of cell viability. The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant, peptide substrate (Gly-Phe-AFC). The substrate enters intact cells, where it is cleaved by the live-cell protease activity to generate a fluorescent signal proportional to the number of living cells. The live-cell protease becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium. (description adapted from vendor Promega)
subclass of: assay kit

HEPATOPAC® co-culture kit
type: Class
IAO_0000118: HepatoPac® micropatterned co-culture
IAO_0000118: HepatoPac®
subclass of: assay kit
IAO_0000118: HepatoPac kit
submitted by: SEED Project - Pistoia Alliance